Fibroblasts are the most common connective tissue cells. They produce the structural framework for animal tissues, synthesize the extracellular matrix and collagen, and play a critical role in wound healing. However, during the cellular aging process, fibroblasts lose their ability to contract, leading to stiffness due to reduced connective tissues.
A study from the Mechanobiology Institute at the National University of Singapore has shown that these fibroblasts can be rejuvenated, or redifferentiated, by being geometrically confined on micropatterns.
The presence of more contractile proteins (in red) in the rejuvenated fibroblasts indicates that they have recovered their ability to contract. These rejuvenated cells were observed to have reduced DNA damage and enhanced cytoskeletal gene expression.
The results of this study were first published in the Proceedings of the National Academy of Sciences on 29 April 2020.
The research team believes that their mechanical reprogramming approach can overcome the shortcomings of conventional rejuvenation methods, including the generation of short-lived or oncogenic fibroblasts.
These mechanically rejuvenated fibroblasts could potentially be used as clinical implants in regenerative medicine and stem cell engineering.
Source:
Journal reference:
Roy, B., et al. (2020) Fibroblast rejuvenation by mechanical reprogramming and redifferentiation. PNAS. doi.org/10.1073/pnas.1911497117.
Machine learning reveals age-related decline in stemness across human tissues